Figure 1.

Morphological Features of hMuStem Cells and Myoblasts

(A) 7 days after the end of the isolation protocol, phase contrast microscopy revealed that hMuStem cells formed a colony unit composed of round and thin cells (arrow) as well as short spindle-shaped cells. A part of the cells remained in the supernatant as floating cells, corresponding to small and highly refractile cells positioned above adherent cells (arrowhead in insert). (B) Typical lengthened and large spindle-shaped cells were observed in myoblast-derived primary culture. (C) hMuStem-cell-derived primary culture was characterized by a large majority of thin elongated cells aligned in networks (arrowhead) and a permanent presence of some refractile round cells (arrow). (D) Monolayer of spindle-shaped cells and thick multinucleated cells characterized the myoblast-derived primary cultures. Scale bars, 100 μm.