Fig. 5.

M7824 increases TRAIL-mediated lysis of human urothelial cancer cells. HTB-4 cells were treated with M7824, M7824mut, anti-PD-L1 or the IgG1 isotype control MAb (20 ug/ml) for 3 days. Cells were then harvested, washed, and incubated with several concentrations of TRAIL for 14 hours. Values indicate mean±SEM from one representative experiment performed in triplicate. Experiment performed three times with similar results. P values <0.05 were considered significant; * P < 0.05, ** P < 0.01. Red stars indicate the significance of M7824 and purple stars the significance of M7824mut vs the isotype control.