Fig. 6.

M7824 increases CEA-specific T-cell mediated lysis of human urothelial cancer cells. HTB-4 cells were treated with M7824, M7824mut, anti-PDL-1, or IgG1 isotype control MAb (20 ug/ml) for 3 days. Cells were harvested, washed, labeled with ¹¹¹In and co-incubated with CEA-specific CD8⁺ T cells (at effector:target ratio of 25:1) for 14 hours. Supernatants were harvested and ¹¹¹In release measured. % tumor lysis = [(experimental cpm-spontaneous cpm)/(maximum cpm-spontaneous cpm)] × 100. Bars indicate mean±SEM. Statistical analyses were done with unpaired t test. P values <0.05 were considered significant. Experiment performed two times with similar results.