Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Clin Microbiol Infect. 2017 Sep 4;24(5):547.e1–547.e8. doi: 10.1016/j.cmi.2017.08.029

Figure 1. Effects of AMPs on Pseudomonas aeruginosa biofilm growth on polarized human airway epithelial (HAE) cells and transepithelial electrical resistance (TEER).

Figure 1

Using a biotic biofilm assay, P. aeruginosa was co-cultured with primary human bronchial epithelial (HBE) cells to examine the ability of WLBU2, compared to LL37, to prevent biofilm formation. Bacteria were allowed to attach to HBE cells for 1h prior to addition of 16µM peptide (A). Peptides, at 32µM (B) and 64µM (C) in PBS, were added to the apical compartment of the cultured HBE cells maintained under ALI condition. TEER was measured at multiple time points to assess recovery time of the ex-vivo epithelium. Results are mean ± SEM from three independent experiments; *p < 0.05, **p < 0.01 for comparison between groups.