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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Cell Immunol. 2017 Sep 14;323:9–18. doi: 10.1016/j.cellimm.2017.08.008

Fig. 5.

Fig. 5

Cytokine expression by allogeneic T cells co-cultured with DCreg. (A) Intracellular cytokine expression by allogeneic CD4+ (upper panels) and CD8+ T cells (lower panels) stimulated with DCreg or matDC generated from bead-isolated monocytes. CFSE-labeled allogeneic pan T cells were co-cultured for 5 days with DC (1DC:10T cells). The DC-stimulated T cells were harvested, then stimulated for 5 h with αCD2,CD3,CD28 microbeads in the presence of Golgi-plug. (B) Cytokine production in MLR cultures of allogeneic T cells stimulated with DC populations generated from bead-isolated monocytes (n=5 individuals; upper panels) or elutriated monocytes (n=7 individuals; lower panels). Data are means ±1SEM. †, p<0.05.