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. Author manuscript; available in PMC: 2019 Mar 10.
Published in final edited form as: J Control Release. 2018 Feb 2;273:147–159. doi: 10.1016/j.jconrel.2018.01.027

Figure 7.

Figure 7

Antigen recall measurements of (A, D) interferon (IFN)-γ, (B, E) interleukin (IL)-2, and (C, F) tumor necrosis factor (TNF)-α production by splenocytes isolated from C57Bl/6 mice on Day 28 post vaccination. Mice were immunized with (A–C) murabutide (MB) microparticles (MPs), soluble ovalbumin (solOVA), soluble MB (solMB), empty MPs (EMPs), Alhydrogel (alum), or PBS, or (D–F) ovalbumin (OVA) microparticles (MPs), monophosphoryl lipid A (MPL), soluble OVA (solOVA), aluminum (alum), empty MPs (EMPs), or PBS on days 0 and 21. Statistical significance with respect to EMPs is presented as *p < 0.05 and **p < 0.005. Statistical significance with respect to solOVA is presented as †p < 0.05 and ††p < 0.005. Data are presented as mean ± standard error of the mean (n = 4).