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. Author manuscript; available in PMC: 2019 Feb 26.
Published in final edited form as: Biochem Biophys Res Commun. 2018 Feb 8;497(1):220–225. doi: 10.1016/j.bbrc.2018.02.058

Figure 4. Inhibition of Piezo1 using GsMTx4.

Figure 4

HCAECs were pre-incubated with vehicle control or the Piezo1 channel inhibitor, GsMTx4 (10 µM), for 30 min prior to stimulation with Yoda1 (1.5 µM) for 5 min. Immunoblotting was then performed on cell lysates using antibodies against phosphorylated Akt (S473) and total Akt to assess the level of Akt activation (A) or against phosphorylated ERK1/2 (T202/Y204) and total ERK1/2 to assess the level of ERK1/2 activation (B). Representative blots from 3 independent experiments are shown. Bar graphs represents the quantification as fold change in the ratio of phosphorylated Akt to total Akt (A) or phosphorylated ERK1/2 to total ERK1/2 (B) compared to the control static condition, which is set to 1. The error bars indicate SE. *P<0.05; **P<0.01; ***P<0.001.