Table 1.
Ion channel currents in hiPSC-CMs (D1).
| Channel current | Ctr (pA/pF) | Blocker (pA/pF) | Sensitive current (pA/pF) | At mV | n | |
|---|---|---|---|---|---|---|
| INa | (Days 30–40) | −30 ± 27.6 | −5.9 ± 5.5 | −23.7 ± 22.1 | −40 | 7 |
| (Days 50–60) | −52.8 ± 44.0 | −10.5 ± 3.6 | −42.3 ± 35.1 | −40 | 6 | |
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| ICa-L | (Days 30–40) | −9.7 ± 1.1 | −3.2 ± 0.6 | −6.4 ± 1.2 | 10 | 10 |
| (Days 50–60) | −13.2 ± 3.8 | −4.4 ± 1.2 | −8.8 ± 2.5 | 10 | 10 | |
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| ICa-T | (Days 30–40) | −2.1 ± 0.8 | −1.0 ± 0.6 | −1.1 ± 0.3 | −30 | 7 |
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| If | (Days 30–40) | −7.5 ± 0.9 | −6.4 ± 0.9 | −1.2 ± 0.3 | −120 | 8 |
| (Days 50–60) | −3.4 ± 0.8 | −1.8 ± 0.4 | −1.6 ± 0.5 | −120 | 5 | |
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| INCX | (Days 30–40) | −6.1 ± 1.0 | −3.7 ± 0.8 | −2.4 ± 0.6 | −85 | 9 |
| (Days 50–60) | −9.6 ± 1.5 | −5.5 ± 1.0 | −4.2 ± 0.8 | −85 | 15 | |
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| IK1 | (Days 30–40) | −13.3 ± 4.0 | −12.5 ± 4.0 | −0.7 ± 0.2 | −120 | 7 |
| (Days 50–60) | −13.4 ± 0.9 | −11.5 ± 0.9 | −1.9 ± 0.4∗ | −120 | 8 | |
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| Ito | (Days 30–40) | 13.0 ± 2.1 | 6.9 ± 1.5 | 6.1 ± 1.0 | 70 | 14 |
| (Days 50–60) | 10.7 ± 2.1 | 5.7 ± 1.3 | 5.0 ± 0.9 | 70 | 10 | |
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| IKr | (Days 30–40) | 2.6 ± 0.4 | 1.8 ± 0.3 | 0.9 ± 0.2 | 40 | 8 |
| (Days 50–60) | 2.5 ± 0.4 | 1.6 ± 0.3 | 1.0 ± 0.2 | 40 | 7 | |
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| IKs | (Days 30–40) | 1.7 ± 0.5 | 0.9 ± 0.3 | 0.8 ± 0.5 | 40 | 15 |
| (Days 50–60) | 1.3 ± 0.4 | 0.5 ± 0.2 | 0.8 ± 0.3 | 40 | 10 | |
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| IKATP | (Days 30–40) | −4.1 ± 0.4 | −2.2 ± 0.8 | −1.9 ± 0.4 | −120 | 5 |
| (Days 50–60) | −4.7 ± 0.9 | −2.4 ± 0.8 | −2.2 ± 0.4 | −120 | 5 | |
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| IKNa | (Days 30–40) | 2.2 ± 0.2a | 2.1 ± 0.2b | 0.1 ± 0.1 | 60 | 8 |
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| IK-pH | (Days 30–40) | 7.0 ± 2.6c | 5.1 ± 2.8d | 1.8 ± 0.8 | 60 | 5 |
| (Days 50–60) | 12.7 ± 3.9c | 9.3 ± 3.0d | 3.4 ± 2.1 | 60 | 4 | |
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| SK1–3 | (Days 30–40) | 1.9 ± 0.7 | 1.5 ± 0.5 | 0.5 ± 0.4 | 40 | 10 |
| (Days 50–60) | 3.1 ± 1.5 | 2.5 ± 1.2 | 0.6 ± 0.3 | 40 | 7 | |
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| IKACh | (Days 30–40) | −6.6 ± 0.3 | −4.09 ± 0.6 | −2.6 ± 0.6 | −120 | 6 |
| (Days 50–60) | −6.7 ± 0.3 | −4.5 ± 0.3 | −2.2 ± 0.3 | −120 | 6 | |
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| ITRPV1 | (Days 30–40) | 3.8 ± 1.4 | 2.4 ± 1.1 | 1.4 ± 0.5 | 40 | 5 |
| (Days 50–60) | 3.5 ± 1.4 | 2.7 ± 1.1 | 0.8 ± 0.4 | 40 | 5 | |
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| ICl-Ca2 | (Days 30–40) | 4.3 ± 1.3 | 3.7 ± 0.8 | 0.6 ± 0.8 | 50 | 6 |
| (Days 50–60) | 4.7 ± 1.2 | 4.0 ± 0.9 | 0.7 ± 0.4 | 50 | 7 | |
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| ISK4 | (Days 30–40) | 5.8 ± 0.8 | 3.0 ± 0.7 | 2.8 ± 0.4 | 50 | 5 |
| (Days 50–60) | 2.6 ± 0.6 | 1.5 ± 0.3 | 1.1 ± 0.9∗ | 50 | 5 | |
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| ICl-vol | (Days 30–40) | 0.8 ± 0.2e | 1.1 ± 0.5f | 0.3 ± 0.5 | 60 | 6 |
| (Days 50–60) | 0.9 ± 0.2e | 2.2 ± 0.6f | 1.2 ± 0.6∗ | 60 | 6 | |
∗ p < 0.05 versus days 30–40; a[Na+]i = 6 mM; b[Na+]i = 50 mM; cpH = 7.4; dpH = 6.0; eisotonic solution; fhypotonic solution.