Table 16.1.

Use of Gal4-UAS system in AMPK research

Expression	Gal4 driven	Transgene	Study
Ubiquitous	Tubulin1	AMPK-α-DN AMPK-RNAi (α, γ) AMPK-α	Effect of AMPK on lethality
	Actin1,6	AMPK-α-DN AMPK-RNAi (α, γ) AMPK-α	Effect of AMPK on lethality
		AMPK-α-RNAi	Effect of AMPK on NDPK activity
	Ubiquitin1,5	AMPK-α-DN AMPK-γ-RNAi	Effect of AMPK on life span and feeding behavior
		AMPK-α (± mCh) truncated AMPK-α (± mCh)	Identification of nuclear export signal in AMPK-α
	Hsp701,a	AMPK-α-DN AMPK-RNAi (α, γ)	Effect of AMPK on life span during starvation
Panneuronal	ELAV4,b	AMPK-γ truncated AMPK-γ	Effect of WT or mutated AMPK-γ on vacuolar pathology in the brain
	ELAV GS3,c	AMPK-α (± mCh)	Effect of neuronal AMPK overexpression on life span (± starvation), intestinal aging, and the expression of autophagy related genes (ATG)
Intestinal	TIGS-2 GS3, c	mCh-AMPK-α	Effect of intestinal AMPK overexpression on intestinal aging, life span
Fat body, digestive system	S106 GS2,7, c	AMPK-α-RNAi	Role of AMPK in mediating life span extension induced by β-sitosterol
Sensory neurons	109(2)801,5	Actin-GFP, AMPK-α-DN AMPK-RNAi (α, γ) truncated AMPK-α	Effect of AMPK on neuronal morphology

^aHeat shock protein (Hsp)70-Gal4 is temperature controllable (McGuire et al. 2004)

^bELAV, embryonic lethal abnormal vision promoter (McGuire et al. 2004) is pan-neuronal

^cGS Gene-Switch Gal4s that are expressed after feeding flies with RU486 (Osterwalder et al. 2001). mCh mCherry fluorescent tag.

⁽¹⁾Johnson et al. (2010),

⁽²⁾Lin et al. (2014),

⁽³⁾Ulgherait et al. (2014),

⁽⁴⁾Tschape et al. (2002),

⁽⁵⁾Kazgan et al. (2010),

⁽⁶⁾Onyenwoke et al. (2012),

⁽⁷⁾Poirier et al. (2008)