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. 2018 Feb 27;10:9. doi: 10.3389/fnagi.2018.00009

Figure 5.

Figure 5

TM-pretreatment reduces the ubiquitination of TRAF6 and decreases nuclear translocation of NFκB by inhibiting the degradation of IκB-α in LPS-induced BV-2 cells. After treated with TM and LPS, cell lysates were collected and immunoprecipitated with TRAF6 antibody, and the IP sample was detected with ubiquitin antibody. (A,B) Immunoblot images (A) and quantifications (B) show that TM-pretreatment lead to the decrease of TRAF6 ubiquitination. (C) ROS was detected by fluorescent enzyme analyzer. (D,E) Immunoblot images (D) and quantifications (E) show that IκB-α expression was inhibited by LPS, but the inhibition was blocked by pre-treatment with TM. (F) Immunoblot images show that TM prohibited the nucleus localization of NFκB induced by LPS. The results are represented as the mean ± SD of at least three independent experiments (N ≥ 3). *p < 0.05 compared with the LPS; #p < 0.05 compared with the control. The p-values were calculated by One-way ANOVA followed by Bonferroni's post-hoc test.