Figure 4.

P. aeruginosa gene expression kinetics in BWE. Expression kinetics measured in BWE and LB pH 7.0 conditions at different OD₆₀₀ values using qRT-PCR on the following genes: (A) alkaline protease (aprA) and exotoxin-A (toxA); (B) clpV1, clpV2, and clpV3 ATPases for H1-, H2, and H3-T6SS, respectively; (C) oprJ for MexCD-OprJ system and opmD for MexGHI-OpmD. Statistics analyses are indicated with P-values. Comparison between similar time points in different culture media is indicated using ^*P < 0.05 and ^**P < 0.01. Comparison between times points in a same culture condition is indicated using #P < 0.05 and ##P < 0.01.