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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Brain Res Bull. 2017 Nov 7;137:23–34. doi: 10.1016/j.brainresbull.2017.11.002

Figure 5. Dose-Dependent Effects of PIREN Pretreatment on ACTH in PHYSO-Stimulated Male and Female Rats.

Figure 5

Response curves of absolute plasma ACTH (A–F) and corresponding percent changes from baseline (G, H). Baseline blood samples were collected at −25 min and −15 min to yield an average baseline at −20 min. Injection of PIREN (10, 30, or 70 mg/kg) or SAL (1 ml/kg) occurred immediately after collection of the −25 min blood sample. Injection of either PHYSO (0.1 mg/kg) or SAL (1 ml/kg) occurred at 0 min. Four additional blood samples were collected at 10 min, 20 min, 40 min, and 60 min. Each bar represents the mean ± SEM. n = 23 to 24 rats for SAL + SAL groups; n = 7 to 8 rats for SAL + PHYSO groups; n = 8 to 13 rats for PIREN pretreatment groups. Females are represented by squares and males are represented by circles. a, sex difference at indicated dose and time point (p < 0.05); b, vs. baseline (p < 0.05); c, vs. SAL + SAL at indicated time point (p < 0.05); d, vs. SAL + PHYSO at indicated time point (p < 0.05). * designates that letters apply to both sexes at indicated time point.