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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Neurochem Int. 2018 Jan 3;114:33–41. doi: 10.1016/j.neuint.2017.12.012

Figure 4.

Figure 4

Repeated stimulations with nicotine. (A) The effect of repeated stimulations, 5 min apart, on the concentration of dopamine measured with 1.8 fmol or 8.8 fmol nicotine stimulation (2-way ANOVA, significant main effects of amount of nicotine and stimulation number, p = 0.0011 for nicotine concentration, p < 0.0001 for stimulation number, and no significant interaction, p = 0.97, n = 6). 8.8 fmol nicotine stimulation evokes more dopamine than 1.8 fmol nicotine (Sidak’s post-test, p < 0.05 for all). (B) Normalized current for repeated stimulations, which also shows main effects of nicotine amount and stimulation number on the response (2-way ANOVA, p < 0.0001 for stimulation number and p < 0.01 for nicotine dose, interaction p < 0.001). There is a greater increase with 1.8 fmol than 8.8 fmol nicotine (Sidak’s post-test), which suggests that the increase in sensitivity to nicotine occurs faster and reaches maximal level faster with higher amounts of nicotine.