Table 1.

Intra- and Inter- Precision and Accuracy

QC Level	Intra - Assay Precision and Accuracya					Inter - Assay Precision and Accuracyb
	Mean [ng/ml]	SD [ng/ml]	%CV	%DEV	Mean [ng/ml]	SD [ng/ml]	%CV	%DEV
	TAF
LLOQ [0.030 ng/mL]	0.0307	0.00410	13.3	2.39	0.0321	0.00450	13.9	7.04
Low [0.090 ng/mL]	0.0846	0.00850	10.0	-5.96	0.0881	0.0127	14.4	-2.07
Mid [3.00 ng/mL]	3.11	0.230	7.38	3.56	3.22	0.200	6.15	7.43
High [130 ng/mL]	120	8.60	7.20	-7.95	123	10.7	8.65	-5.13
	TFV
LLOQ [1.00 ng/mL]	1.03	0.100	9.64	3.27	1.01	0.107	10.6	0.680
Low [3.00 ng/mL]	2.91	0.320	11.0	-3.00	2.80	0.269	9.58	-6.52
Mid [20.0 ng/mL]	18.6	1.10	5.93	-7.25	18.5	1.05	5.68	-7.50
High [170 ng/mL]	174	5.06	2.91	2.35	172	7.99	4.66	0.980
	CMX157
LLOQ [0.25 ng/mL]	0.265	0.0290	11.1	6.07	0.269	0.0310	11.4	7.76
Low [0.75 ng/mL]	0.735	0.0480	6.54	-1.98	0.734	0.0540	7.42	-2.13
Mid [50.0 ng/mL]	52.1	2.34	4.50	4.17	50.1	2.39	4.76	0.211
High [160 ng/mL]	171	5.32	3.10	7.08	167	5.87	3.52	4.31

^an=6 for each level of QC; representative data from a single analytical run

^bn=18 for all QC levels; complex precision and accuracy from three analytical runs