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. 2018 Feb 28;8:56. doi: 10.3389/fcimb.2018.00056

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Copyright © 2018 Kumar, Sorensen and Cardona.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of BCAL1069 (cdpA) motility in swimming motility. (A) The swimming motility of the strains was examined in semi-solid SCFM 0.3% agar plates at 37°C after 24 h. The motility assay was performed three-times independently in duplicates (^*p < 0.01). (B) To detect flagellin protein, proteins from whole cell lysates of strains were separated on 12% SDS-PAGE followed by flagellin detection using anti-flagellin primary antibody and alkaline phosphatase cross linked to secondary antibody. Western blots were performed twice. One representative experiment is shown. (C) The presence of flagella in the WT and mutant strains were observed using transmission electron microscope.