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. 2018 Feb 28;9:360. doi: 10.3389/fmicb.2018.00360

Figure 5.

Figure 5

EA internalization is altered by the expression of activation mutated Rho-family GTPases. HeLa cells transfected with WT, DN or CA mutated GTPases were incubated with EAs (MOI 10) for 2 h, and intracellular parasites were quantified under epifluorescence microscope in transfected cells. Cells transfected with cytosolic GFP (GFP) were used as a control group and displayed similar EA internalization as non-transfected cells (-). (A) Cells expressing CA-Rac1 displayed increased EA internalization, and cells expressing DN-Rac1 displayed reduced EA internalization compared to the GFP control group. (B) For Cdc42, cells expressing WT construct had increased EA internalization and cells expressing CA construct had reduced EA internalization compared to the GFP control group. (C) Cells transfected with WT-RhoA or CA displayed reduced EA internalization compared to the GFP control group. Results represent mean of two independent experiments in triplicate or duplicate, and each dot represents one replicate (One-Way ANOVA, ***P < 0.001, **P < 0.01, n ≥ 4).