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. 2018 Feb 28;6:17. doi: 10.3389/fbioe.2018.00017

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Copyright © 2018 Hattab, Wiesmann, Becker, Munzner and Nattkemper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binary images annotated with computed particle positions (shown as red circles). (A) Original biomovie D1 binary image. (B) Simulated movie binary image. (C) Original biomovie crop of D1 showing 1–2 particles detected within each cell. A particle diameter value of d = 9 px yields no false negatives, and some false positives that will be eliminated in subsequent processing that exploits temporal coherence. (D) Simulated movie crop showing ~2 particles detected per cell, with a particle diameter d = 17 px.