Skip to main content
. Author manuscript; available in PMC: 2018 Mar 5.
Published in final edited form as: Science. 2017 Sep 1;357(6354):886–891. doi: 10.1126/science.aan3174

Fig. 4. The signaling relay may serve to delay differentiation to ensure consistent column assembly.

Fig. 4

(A–D) Early pupal (stages indicated) eye-optic lobe complexes stained for Elav (yellow), Dac (magenta), HRP (cyan) and (C, D) GFP (white). Cyan dashed line marks the youngest photoreceptors. (A) Control. (B) rho3−/−. (C) An early-onset pan-photoreceptor Gal4 driving GFP and Ilp6 in a rho3−/− background. Differentiation was widespread and initiated in the youngest column (arrowhead), which contained ~4 lamina precursors. (D–E) A late-onset pan-photoreceptor Gal4 driving GFP and Ilp6 in a rho3−/− background. (D) At ~10hrs APF, differentiation initiated only in old columns (arrowheads), but columns assembled 6–7lamina precursors/column. (E) At ~15hrs APF, GFP and Ilp6 were expressed in all photoreceptors. Differentiation was widespread but variable as some columns contained more differentiated neurons than their older neighbors (arrowhead). (Scale bar = 10µm).