Fig. 4.

Allergic conjunctivitis increased the expression of myopia-related tissue-remodeling proteins. (a) Immunohistochemical analyses of TGF-β, MMP2, and COL-I in negative, PBS-treated, and OVA-treated eyes are shown. (b–d) The relative expression levels of TGF-β (b), MMP2 (c), and COL-I (d) were determined with the Image J software (Schindelin et al., 2012). (e) mRNA expression levels of TGF-β, MMP2, and COL-I in the retinas of PBS and OVA groups were compared to those in the negative group. ANOVA was used to determine significant differences (p < 0.0001) and Sidak's multiple comparisons tests were used for paired comparisons between PBS- and OVA-treated eyes. P < 0.05 was considered statistically significant. *: indicates a significant difference.