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. 2018 Jan 31;28:274–286. doi: 10.1016/j.ebiom.2018.01.024

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© 2018 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 7 — Expression levels of inflammation-related transcription factors and cytokines in allergic conjunctivitis eyes. (a) Immunohistochemical analyses of TNF-α, IL-6, IL-8, MCP-1, and IL-10 expressions in negative, PBS-treated, and OVA-treated eyes. (b) The relative expression levels of TNF-α, IL-6, IL-8, MCP-1, and IL-10 were determined with the Image J software. (c) mRNA expression levels of TNF-α, IL-6, IL-8, MCP-1, and IL-10 in the retinas of the PBS and OVA groups were compared to those in the negative group. ANOVA was used to determine significant differences (p < 0.0001) and Sidak's multiple comparisons test was used for paired comparisons between PBS- and OVA-treated eyes. P < 0.05 was considered statistically significant. *: indicates a significant difference. (d) Immunohistochemical analyses of NFκB and IκB expressions in negative, PBS-treated, and OVA-treated eyes. (e) The relative expression levels of NFκB and IκB were determined with the Image J software. (f) mRNA expression levels of NFκB and IκB in the retinas of the PBS and OVA groups were compared to those in the negative group. ANOVA was used to determine significant differences (p < 0.0001) and Sidak's multiple comparisons test was used for paired comparisons between PBS- and OVA-treated eyes. P < 0.05 was considered statistically significant. *: indicates a significant difference.