EGFR inhibitor abrogates the up-regulation of PD-L1 caused by RT in glioma cells.
EGFR signaling and PD-L1 protein level in U87 and U251 cell lines were detected at 24, 48 and 72 h after 5 or 10 Gy irradiation. Before irradiation, U87 and U251 cells were incubated in the presence of 10 μM AG490 at 37 °C for 1 h. Each column is shown as the means of three separate experiments; bars, SD. ANOVA; *P < 0.05; **P < 0.01; ***P < 0.001; versus 0 Gy group.