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. 2018 Feb 28;9:212. doi: 10.3389/fpls.2018.00212

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Copyright © 2018 Shi, Cao, Chen, Chen, Zheng and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enzymatic assay of recombinant MrLAR using leucocyanidin as a substrate. (A) Analysis of MrLAR protein on a 12.5% SDS-PAGE gel. M, Molecular weight marker; 1, crude protein from E. coli BL21 (DE3) harboring pET32α-MrLAR induced by IPTG; 2, purified recombinant MrLAR protein. (B–D). LC-MS [M-H]⁻ extracted ion chromatographs (m/z = 289) of authentic (+)-catechin (C) and (−)-epicatechin (EC) (B) along with the enzymatic products of purified recombinant MrLAR protein (C) or protein extract from vector control (D).