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E2F1 activates CCAT2 transcription. A. The relative expression level of CCAT2 in control and E2F1-silencing cells was detected by RT-PCR. B. The binding of E2F1 and CCAT2 promoter was detected by ChIP assay. C. Luciferase assays of the cells indicated that were transfected with pGL3, pGL3-CCAT2, or pGL3-CCAT2-mut vectors, the E2F1 vector, or an empty vector. Error bars indicate mean ± standard errors of the mean. *P < 0.05.
