Figure 3.

CCAT2 enhances cell proliferation, induced cell cycle progression and inhibits cell apoptosis in pituitary adenomas cells. A. The relative expression of CCAT2 in control and CCAT2-knockdown cells was detected by RT-PCR. B. Growth curves for HP75 cells after transfection with CCAT2 shRNA or the negative control were determined by CCK-8 assays. C. The relative expression of CCAT2 in control and CCAT2-overexpressing cells was detected by RT-PCR. D. Growth curves for HP75 cells after transfection with CCAT2 or empty vector were determined by CCK-8 assays. E. Cells in S-phase population were significantly decreased when CCAT2 was silenced in HP75 cells. F. FACS analysis showing significant increases or decreases of cells in S- or G-1 phase, respectively, in HP75 cells overexpressing CCAT2. G. Cells with silenced CCAT2 expression were stained with a combination of annexin V and 7-AAD and analyzed by FACS. Cells positive for annexin V staining were counted as apoptotic cells, and the percentage of apoptotic cells is shown. H. Cells with CCAT2 overexpression were stained with a combination of annexin V and 7-AAD and analyzed by FACS. Error bars indicate mean ± standard errors of the mean. *P < 0.05.