Table 2. Overview of strategies for P450 production in E. coli. Where plural strategies have been tested, the optimal one is marked in bold.

P450	N-terminal modification	Coexpression	Volumetric titer	Yield	Reference
CYP4B1	MALLLAVF	–	660 nmol/l		[52]
CYP2E1	–	–	900–1400 nmol/l	14 nmol/mg protein	[53]
CYP154E1	–	Pdx/PdR and YkuN/FdR	825 nmol/l		[45]
CYP51F1	Truncation (2–37)	GroEL/GroES	1255 nmol/l		[24]
CYP61A1	Truncation (2–36)	GroEL/GroES	761 nmol/l		[24]
CYP505D6	–	GroEL/GroES	665 nmol/l		[24]
CYP505D8v1	–	GroEL/GroES	1333 nmol/l		[24]
CYP512E1	Truncation (2–8)	GroEL/GroES	973 nmol/l		[24]
CYP5137A4v1	–	GroEL/GroES	1820 nmol/l		[24]
CYP5139D7v1	Truncation (2–13)	GroEL/GroES	1230 nmol/l		[24]
CYP5147B1	Truncation (2–8)	GroEL/GroES	1310 nmol/l		[24]
CYP5150A2	Truncation (2–14)	GroEL/GroES	1020 nmol/l		[24]
CYP5037B3	NTD of CYP5144C1	GroEL/GroES	1213 ± 53 nmol/l		[24]
CYP5037E1v1	NTD of CYP5144C1	GroEL/GroES	2330 ± 44 nmol/l		[24]
CYP5146A1	NTD of CYP5144C1	GroEL/GroES	1041 ± 118 nmol/l		[24]
CYP5149A1	NTD of CYP5144C1	GroEL/GroES	2172 ± 62 nmol/l		[24]
CYP5037D1v1	NTD of CYP5144C1	GroEL/GroES	1213 nmol/l		[25]
CYP5037D1v2	NTD of CYP5144C1	GroEL/GroES	1440 nmol/l		[25]
CYP5037E1v2	NTD of CYP5144C1	GroEL/GroES	1645 nmol/l		[25]
CYP5147A4	NTD of CYP5144C1	GroEL/GroES	1552 nmol/l		[25]
CYP2B4	–	–	812 nmol/l	17 nmol/mg protein	[54]
CYP2B1	N-terminal truncation (3–21), positive charges substituted	–	800–1000 nmol/l	19 nmol/mg protein	[55]
CYP2C9	Truncation	CPR	800 nmol/l		[39]
CYP1A2	Truncation	CPR	1010 nmol/l		[39]
CYP2C3	N-terminal truncation (3–20)	–	800–1200 nmol/l	17 nmol/mg protein	[56]
CYP2W1	Various N-terminal modification, rabbit P450 sequence (MAKKTSSKGK)	GroEL/GroES	1800 nmol/l	16.8 nmol/mg protein	[48]