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. 2018 Apr 13;38(2):BSR20171386. doi: 10.1042/BSR20171386

Figure 8. LiCl attenuated the inhibitive effect of β-elemene on the proliferation and canonical Wnt/β-catenin pathway of primary human airway granulation fibroblasts.

Figure 8

(A) Primary human airway granulation fibroblasts were cultured in media containing 2% FBS and 160 μg/ml β-elemene in the presence or absence of 10 mM LiCl for 48 h. The morphology of cells was shown in photomicrographs (200× magnification). (B) The proliferation rate of cells was assessed by MTT assay. (C) The mRNA levels of Wnt3a, GSK-3β, β-catenin, α-SMA, TGF-β, and Col-I were assessed by QRTPCR. (D) The protein levels of Wnt3a, GSK-3β, phospho-GSK-3β (Ser9), β-catenin, and α-SMA were assessed by Western blot. (E) The protein levels of TGF-β and Col-I were assessed by ELISA. GAPDH was used as the internal control and the data of three independent experiments were expressed as the mean ± SD (*P<0.05, **P<0.01).