FIGURE 4.
Mixed bacterial population and Phage Cocktails. Similar to EFDG1r bacteria; when the naïve E. faecalis V583 and mutant EFDG1r are mixed in equal concentrations to get a mixed population; the growth curve (A) for logarithmic and (B) for stationary phase bacterial mixtures and their CFU/ml count counterparts (C) and (D) logarithmic and stationary respectively show desirable lysis by EFLK1 phage and its 1:1 phage cocktail. While EFDG1 phage has no significant effect in either case. (E) Crystal violet staining reveals a reduction in biofilm biomass and (F) CFU/ml also shows reduction in bacterial counts when treated with EFLK1 phage and phage cocktail.