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Verification of RNA-Seq analysis by qRT-PCR. (A) Relative expression levels of 12 randomly selected transcripts are verified through qRT-PCR. The gray histograms represent the relative gene expression levels analyzed using qRT-PCR. The black histograms represent TMM-FPKM values which are the relative expression levels of RNA-Seq data. The error bars are the mean ± SE of three replications. (B) Comparison between the log2 of expression ratios of DGEs obtained from RNA-Seq and qRT-PCR.
