FIG 3.
Accumulation of 4-HBA and shikimate in a 24-h batch culture of metabolically engineered C. glutamicum strains, using a baffled flask. CRZ23, the basal strain for the production of 4-HBA without overexpression of the gene ubiC (genotype is shown in Table 3); PHE261, the strain overexpressing ubiC from P. rustigianii; PHE262, the strain overexpressing one additional aroGEC(S180F); PHE263, the strain overexpressing aroCKB; PHE264, the strain overexpressing aroD, aroA, and aroE; PHE156, the strain overexpressing two additional aroCKB genes; PHE340, the strain that shares the same genotype with PHE156 except for the overexpression of ubiC from C. sakazakii; and PHE283, the strain that shares the same genotype with PHE156 except for the overexpression of ubiC from E. coli. The average values of the results from triplicate experiments are shown. Error bars indicate standard deviations.