TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| C. glutamicum | ||
| R | Wild-type strain | 43, JCM18229 |
| ACX-araE | R with markerless integration of xylA, xylB, bglF317A-bglA, araBAD, and araE | 44 |
| CRZ23 | ACX-araE with ldhA::markerless, qsuB::markerless, qsuD::markerless, pobA::markerless, poxF::markerless, markerless integration of C. glutamicum R tkt-tal and E. coli aroG(S180F) | This study |
| PHE261 | Kmr; CRZ23 harboring Pphe292 | This study |
| PHE262 | Kmr; PHE261 with markerless integration of one more aroG(S180F) (E. coli) | This study |
| PHE263 | Kmr; PHE262 with markerless integration of C. glutamicum R aroCKB | This study |
| PHE264 | Kmr; PHE263 with markerless integration of C. glutamicum R aroD, aroA, and aroE | This study |
| PHE156 | Kmr; PHE264 with markerless integration of two more sets of C. glutamicum R aroCKB | This study |
| PHE340 | Kmr; PHE156 harboring Pphe302 instead of Pphe292 | This study |
| PHE283 | Kmr; PHE156 harboring Pphe17 instead of Pphe292 | This study |
| PHE240 | Kmr; PHE156 with markerless deletion of pyk | This study |
| PHE242 | Kmr; PHE156 with markerless deletion of hdpA | This study |
| PHE243 | Kmr; PHE156 with markerless deletion of pyk and hdpA | This study |
| Plasmids | ||
| pCRB22 | Kmr; E. coli-C. glutamicum shuttle vector derived from pHSG298 and pCASE1; 4.1 kb | 13 |
| Pphe292 | Kmr; pCRB22 with a PgapA-ubiC (P. rustigianii)-term gene; 5.6 kb | This study |
| Pphe302 | Kmr; pCRB22 with a PgapA-ubiC (C. sakazakii)-term gene; 5.7 kb | This study |
| Pphe17 | Kmr; pCRB22 with a PgapA-ubiC (E. coli)-term gene; 5.6 kb | This study |
a
The number in parentheses indicates the position of the altered amino acid residue. PgapA, a promoter of the gapA gene. Kmr, kanamycin resistance.