TABLE 4.
4-HBA production, glucose consumption, and by-product formation of metabolically engineered C. glutamicum strainsa
| Strain | Time (h) | 4-HBA concn (mM)b | Glucose consumption (mM) | Yield (% [mol/mol])c | Concn (mM)d |
|||
|---|---|---|---|---|---|---|---|---|
| Shi | Phe | Pyr | DHA | |||||
| PHE156 | 10 | 111.8 | 289.8 | 39.1 ± 0.5 | 1.5 | 0.5 | 2.6 | ND |
| 24 | 255.7 | 649.1 | 39.4 ± 1.4 | 4.2 | 2.4 | 18.3 | 10.6 | |
| PHE240 | 10 | 110.3 | 273.4 | 40.4 ± 1.3 | 2.4 | 0.2 | 2.3 | 1.0 |
| 24 | 253.6 | 634.4 | 40.0 ± 0.5 | 5.6 | 1.8 | 14.9 | 4.8 | |
| PHE242 | 10 | 112.3 | 273.0 | 41.1 ± 0.7 | 1.0 | 0.1 | 2.1 | ND |
| 24 | 263.4 | 639.4 | 41.2 ± 0.6 | 3.2 | 1.4 | 12.6 | 0.1 | |
| PHE243 | 10 | 113.1 | 275.7 | 41.0 ± 1.5 | 1.1 | 0.1 | 2.0 | ND |
| 24 | 265.4 | 638.5 | 41.6 ± 0.5 | 3.4 | 1.4 | 13.7 | ND | |
a
Data are the means of the results from three experiments, unless otherwise indicated.
b
4-HBA, 4-hydroxybenzoic acid.
c
Yield was calculated as mol 4-HBA produced per mole glucose consumed. Data are means ± standard deviations (SD) (n = 3).
d
Shi, shikimate; Phe, phenylalanine; Pyr, pyruvate; DHA, 1,3-dihydroxyacetone; ND, not detected.