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. 2018 Mar 1;84(6):e02430-17. doi: 10.1128/AEM.02430-17

TABLE 5.

PCR Primers used in this study

Target Primer name Sequence (5′→3′) Reference
blaCTX-M Pan CTX-M forward TTT GCG ATG TGC AGT ACC AGT AA 48
Pan CTX-M reverse CGA TAT CGT TGG TGG TGC CAT A
blaCTX-M (group I) CTXM1-F3 forward GAC GAT GTC ACT GGC TGA GC 59
CTXM1-R2 reverse AGC CGC CGA CGC TAA TAC A
blaCTX-M (group II) TOHO1-2F forward GCG ACC TGG TTA ACT ACA ATC C 59
TOHO1-1R reverse CGG TAG TAT TGC CCT TAA GCC
blaCTX-M (group III) CTXM8.WSAgroupIII.F forward AGA CCT GAT TAA CTA CAA TCC CAT TA 51
CTXM8.WSAgroupIII.R reverse ACT TTC TGC CTT CTG CTC TGG C
blaCTX-M (group IV) CTXM914F forward GCT GGA GAA AAG CAG CGG AG 59
CTXM914R reverse GTA AGC TGA CGC AAC GTC TG
blaCTX-M sequencing primers CTXM-1 upstream forward ATG TTG TTG TTA ATT CGT CTC 46
CTXM-1 upstream reverse CGT TAT CGC TGT ACT GTA G
CTXM-2 downstream forward TTA ACT ATA ATC CGA TTG CG 46
CTXM-2 downstream reverse TTT CTG CCT TAG GTT GAG
blaCTX-M (CTX-M-9 group) M9 upper forward ATG GTG ACA AAG AGA GTG CA 60
M9 lower reverse CCC TTC GGC GAT GAT TCT C
uidA UAL-754 forward AAA ACG GCA AGA AAA AGC AG 47
UAR-900 Reverse ACG CGT GGT TAC AGT CTT GCG