TABLE 5.
PCR Primers used in this study
Target | Primer name | Sequence (5′→3′) | Reference |
---|---|---|---|
blaCTX-M | Pan CTX-M forward | TTT GCG ATG TGC AGT ACC AGT AA | 48 |
Pan CTX-M reverse | CGA TAT CGT TGG TGG TGC CAT A | ||
blaCTX-M (group I) | CTXM1-F3 forward | GAC GAT GTC ACT GGC TGA GC | 59 |
CTXM1-R2 reverse | AGC CGC CGA CGC TAA TAC A | ||
blaCTX-M (group II) | TOHO1-2F forward | GCG ACC TGG TTA ACT ACA ATC C | 59 |
TOHO1-1R reverse | CGG TAG TAT TGC CCT TAA GCC | ||
blaCTX-M (group III) | CTXM8.WSAgroupIII.F forward | AGA CCT GAT TAA CTA CAA TCC CAT TA | 51 |
CTXM8.WSAgroupIII.R reverse | ACT TTC TGC CTT CTG CTC TGG C | ||
blaCTX-M (group IV) | CTXM914F forward | GCT GGA GAA AAG CAG CGG AG | 59 |
CTXM914R reverse | GTA AGC TGA CGC AAC GTC TG | ||
blaCTX-M sequencing primers | CTXM-1 upstream forward | ATG TTG TTG TTA ATT CGT CTC | 46 |
CTXM-1 upstream reverse | CGT TAT CGC TGT ACT GTA G | ||
CTXM-2 downstream forward | TTA ACT ATA ATC CGA TTG CG | 46 | |
CTXM-2 downstream reverse | TTT CTG CCT TAG GTT GAG | ||
blaCTX-M (CTX-M-9 group) | M9 upper forward | ATG GTG ACA AAG AGA GTG CA | 60 |
M9 lower reverse | CCC TTC GGC GAT GAT TCT C | ||
uidA | UAL-754 forward | AAA ACG GCA AGA AAA AGC AG | 47 |
UAR-900 Reverse | ACG CGT GGT TAC AGT CTT GCG |