FIG 3.

Transcription levels of mscCG (A) and mscCG2 (B) of C. glutamicum Z188 under l-glutamate-nonproducing and -producing conditions. C. glutamicum Z188 was cultivated in biotin-rich or biotin-poor fermentation media, and cells were collected during the first 12 h. Three parallel experiments were performed. Total RNA was extracted, treated with DNase I, and used for cDNA synthesis. qPCR was conducted using cDNA as a template and the primers listed in Table 4. Error bars indicate standard deviations of results from three parallel experiments.