FIG 7.

Cell survival rates of C. glutamicum Z188 derivatives upon osmotic downshock. Cells cultivated in a high-osmolarity LBG medium containing 1.17 M NaCl (2,350 mosmol) were transferred into normal LBG medium containing 0.17 M NaCl (410 mosmol) or high-osmolarity LBG medium containing 1.17 M NaCl (2,350 mosmol). The cultures were incubated at 30°C and with shaking at 220 rpm for another 40 min, diluted using isosmotic medium, and plated on solid LBG medium. The viability of the diluted cells was determined by counting the number of colonies on the plates. Stars indicate significant changes in cell survival rates based on a comparison between different strains. *, P ≤ 0.05; **, P ≤ 0.01 (Student's two-tailed t test). Error bars indicate standard deviations of results from three parallel experiments.