TABLE 1.
Effects of mscCG and mscCG2 on l-glutamate excretion in C. glutamicum ATCC 13032a
| Strain | l-Glutamate concn (mM) | Dry cell wt (g/liter) |
|---|---|---|
| ATCC 13032 | 44.2 ± 4.8 | 8.3 ± 0.6 |
| ATCC 13032(pTRCmob) | 39.2 ± 6.9 | 7.1 ± 0.3 |
| ATCC 13032(mscCG) | 42.4 ± 1.4 | 7.1 ± 0.1 |
| ATCC 13032(mscCG2) | 39.2 ± 1.0 | 6.8 ± 0.2 |
| ATCC 13032ΔmscCG(pTRCmob) | 6.8 ± 0.0*** | 7.9 ± 0.2 |
| ATCC 13032ΔmscCG(mscCG) | 42.8 ± 3.7 | 8.1 ± 0.5 |
| ATCC 13032ΔmscCG(mscCG2) | 37.4 ± 4.8 | 8.3 ± 0.0 |
a
Samples were picked and analyzed after 45 h of cultivation in shake flasks. Data represent averages and standard deviations of results of three independent cultures. Asterisks indicate significant changes in extracellular l-glutamate concentration based on a comparison between the engineered strain and wild-type strain ATCC 13032. ***, P ≤ 0.001 (Student's two-tailed t test).