TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| E. coli DH5α | General cloning host | TaKaRa |
| C. glutamicum | ||
| Z188 | l-Glutamate-producing strain | Lab collection |
| ATCC 13032 | Wild-type strain | ATCC |
| Z188(pTRCmob) | Z188 derivative harboring pTRCmob | This study |
| Z188(egfp) | Z188 derivative harboring pTRCmob-egfp | This study |
| Z188(mscCG2-egfp) | Z188 derivative harboring pTRCmob-mscCG2-egfp | This study |
| Z188ΔmscCG2 | Z188 derivative with its mscCG2 deleted | This study |
| Z188ΔmscCG | Z188 derivative with its mscCG deleted | This study |
| Z188ΔmscCG2ΔmscCG | Z188 derivative with its mscCG2 and mscCG deleted | This study |
| Z188ΔmscCG2ΔmscCG(pTRCmob) | Z188ΔmscCG2ΔmscCG derivative harboring pTRCmob | This study |
| Z188ΔmscCG2ΔmscCG(mscCG) | Z188ΔmscCG2ΔmscCG derivative harboring pTRCmob-mscCG | This study |
| Z188ΔmscCG2ΔmscCG(mscCG2) | Z188ΔmscCG2ΔmscCG derivative harboring pTRCmob-mscCG2 | This study |
| ATCC 13032(pTRCmob) | ATCC 13032 derivative harboring pTRCmob | This study |
| ATCC 13032(mscCG) | ATCC 13032 derivative harboring pTRCmob-mscCG | This study |
| ATCC 13032(mscCG2) | ATCC 13032 derivative harboring pTRCmob-mscCG2 | This study |
| ATCC 13032ΔmscCG | ATCC 13032 derivative with its mscCG deleted | This study |
| ATCC 13032ΔmscCG(pTRCmob) | ATCC 13032ΔmscCG derivative harboring pTRCmob | This study |
| ATCC 13032ΔmscCG(mscCG2) | ATCC 13032ΔmscCG derivative harboring pTRCmob-mscCG2 | This study |
| ATCC 13032ΔmscCG(mscCG) | ATCC 13032ΔmscCG derivative harboring pTRCmob-mscCG | This study |
| Plasmids | ||
| pTRCmob | E. coli-C. glutamicum shuttle expression vector with a derepressed Ptrc promoter, Kmr | 40 |
| pK18mobsacB | Gene deletion/insertion vector derived from pK18; mob sacB Kmr | 30 |
| pET21a-egfp | pET21a derivative, carries an enhanced green fluorescent protein-encoding gene, Ampr | 32 |
| pTRCmob-egfp | pTRCmob derivative, carries egfp | This study |
| pTRCmob-mscCG2-egfp | pTRCmob derivative, carries mscCG2-egfp | This study |
| pK18mobsacB-ΔmscCG2 | pK18mobsacB derivative, carries a mutant allele of mscCG2 from Z188 | This study |
| pK18mobsacB-ΔmscCG | pK18mobsacB derivative, carries a mutant allele of mscCG from Z188 | This study |
| pTRCmob-mscCG2 | pTRCmob derivative, carries mscCG2 from Z188 | This study |
| pTRCmob-mscCG | pTRCmob derivative, carries mscCG from Z188 | This study |
| pTRCmob-mscCG2A151V | pTRCmob derivative, carries a mutant of mscCG2 from Z188 | This study |
| pTRCmob-mscCG2A151T | pTRCmob derivative, carries a mutant of mscCG2 from Z188 (mscCG2A151T) | This study |
a
Kmr and Ampr, resistance to kanamycin and ampicillin, respectively.