FIG 3.

Strains selected for whole-genome sequencing span a 25-fold expression range. (A) Workflow from screening to whole-genome sequencing (WGS). Forty-four transformants from the screening pool of 755 transformants were used for dilution streaking and rescreened in biological 4-fold replicates. Twenty-seven strains eventually were used for WGS. The image of the dilution streaking is taken from the Public Health Image Library (identifier 7925), CDC/James Gathany. (B) eGFP fluorescence measurements of the strains selected for WGS span a 25-fold expression range, and the highest-expressing strain surpasses average clones by more than 6-fold. Cells were pregrown on glucose for 60 h and subsequently induced with methanol for 48 h. eGFP reporter fluorescence normalized to cell growth (OD₆₀₀) is shown. Mean values and standard deviations of biological 4-fold replicates are shown. Here, 25 strains transformed with eGFP plasmids are shown, and the parental strain (mut^S) and the mut^S strain transformed without DNA were sequenced. Identifiers refer to internal strain collection numbers assigned at QUT.