TABLE 6.
Primers used in qRT-PCR
| Primer | Oligonucleotide sequence (5′ → 3′) | Lengtha (bp) |
|---|---|---|
| argG-F | AGTGGCATTGGGCTCG | 127 |
| argG-R | ACACCAGCTTCAATCGCA | |
| carA-F | AGAGCATCAAATGAGCCAAC | 112 |
| carA-R | AACGACAACTTTTCTGCCTG | |
| dnaK-F | GACCGTAACACAACTATCCCA | 113 |
| dnaK-R | TCAGCTGCCATTGGACG | |
| uspA-F | GGCACAGAAGTTGATTTTGAG | 113 |
| uspA-R | AGACCAGTTGACCCAATTACA | |
| pyrR-F | GACCGTGGACATCGTGAAT | 119 |
| pyrR-R | AAAATACTGTCATTGCCATCG | |
| purA-F | AGAAATCGCTTATCTCGCTG | 133 |
| purA-R | AATCTTCTTATCGCCTTTGG | |
| mtsB-F | AGGAGAACTATCAGGTGGTCAG | 112 |
| mtsB-R | GCTTATCGAATCAATCCCAA | |
| gltS-F | AGGAGCCGTGATTGAAAAC | 122 |
| gltS-R | TAGGAAGTGCAACCGCTG | |
| tcsK7-F | GAGGAGCTGAAGAATCAGGTT | 125 |
| tcsK7-R | TTCACAAGTGAAGCCATACTCA | |
| nisK-F | TCAATATCTTTGTTAATGCCTGT | 117 |
| nisK-R | AAGGATGACCATTATTCCAGAT | |
| arcA-F | CAGGAATTATGGATGGTGCT | 113 |
| arcA-R | GACTTTCTTGAGTGCTGCTTTA | |
| hrcA-F | TTCTTAATGCTCCTCAACGTG | 105 |
| hrcA-R | TTACTTCACCAGTCCCTAATGTT | |
| epsH-F | TTTTTGGATCATACCCCATAA | 102 |
| epsH-R | TACTTCTTCCGAAAATTTCATG | |
| gapA-F | AATGACCCTTGATGGTCCA | 120 |
| gapA-R | AGTTCAGGCAAAACAAGACC | |
| enoA-F | AATTCCACTCTATCGCTATCTTG | 134 |
| enoA-R | CCCGTTTGACTGGTGTAATC |
a
That is, the length of the qRT-PCR fragment.