TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| SM10 λpir | E. coli conjugation strain | 70 |
| S17-1 λpir | E. coli conjugation strain | 70 |
| EC100D | E. coli cloning strain, pir-116 version | Epicentre |
| K746 | E. coli keratitis isolate | 71 |
| LT2 | S. enterica serovar Typhimurium | 72 |
| 883 | K. pneumoniae, clinical isolate | 73 |
| CHASM | S. marcescens, environmental isolate | 25 |
| K904 | S. marcescens, keratitis isolate | 25 |
| Db11 | S. marcescens, insect isolate | 74 |
| CMS2265 | K904 gummy mutant 1, K904 gumB::Tn | This study |
| CMS4001 | K904 ΔgumB | This study |
| CMS4124 | K904 ΔgumB wza::Tn | This study |
| CMS4225 | K904 fimC-kan | 75 |
| CMS4619 | K904 ΔgumB wecA::Tn | This study |
| CMS4320 | K904 gummy mutant 2, K904 gumB::Tn | This study |
| CMS4462 | Db11 ΔgumB | This study |
| CMS4662 | CHASM ΔgumB | This study |
| Plasmids | ||
| pBT20 | Transposon delivery plasmid | 63 |
| pSC189 | Transposon delivery plasmid | 64 |
| pMQ132 | pBBR1-replicon shuttle vector, aacC-1 | 68 |
| pMQ200 | oriR6K plasmid with PBAD promoter, nptII | 68 |
| pMQ460 | Allelic replacement vector | 76 |
| pMQ480 | pMQ132 plus gumB from S. marcescens strain K904 | This study |
| pMQ507 | pMQ460 plus ΔgumB | This study |
| pMQ530 | pMQ132 plus igaA from S. enterica strain LT2 | This study |
| pMQ529 | pMQ132 plus kumO from K. pneumoniae strain 883 | This study |
| pMQ531 | pMQ132 plus yrfF from E. coli strain K746 | This study |