Figure 3.

The deletion of Mcu does not prevent the degeneration of Purkinje cells lacking AFG3L2. Afg3l2^fl/fl and Mcu^fl/fl were bred with transgenic mice-expressing Cre recombinase under the control of the L7 promoter specifically in Purkinje cells⁶⁸. Cerebelli of 6-week-old offsprings of the indicated phenotypes were stained with calbindin to visualize cerebellar Purkinje cells, IBA1 to mark activated microglia and GFAP for reactive astrocytes (as described in ref.²⁷). The experiments are in agreement with the national ethical guidelines for studies in animals (84-02.04.2015.A402). GCL, granule cells layer; PCL, Purkinje cell layer; ML, molecular cell layer. A representative picture is shown for each condition. Scale bar, 100 μm.