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. 2018 Jan 12;28(3):323–335. doi: 10.1038/cr.2018.2

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Impaired hematopoietic stem cell homeostasis in allergen-induced airway inflammation. (A, B) Representative FACS plots and quantification of HSCs (LSKs, Lin⁻Sca-1⁺c-Kit⁺) from the bone marrow (BM) of the OVA-treated mice (OVA) and controls (NS). (C) Absolute numbers of stem cells at different stages, including LT-HSCs (CD34⁻Flk2⁻LSKs), ST-HSCs (CD34⁺Flk2⁻LSKs) and MPPs (CD34⁺Flk2⁺LSKs). (D) BrdU incorporation assay (24 h) of BM LT-HSCs from the OVA-treated mice (OVA) and controls (NS). (E, F) Absolute numbers of progenitor cells in the BM. CLP, common lymphoid progenitors; GMP, granulocyte/monocyte lineage progenitors; CMP, common myeloid progenitors; MEP, megakaryocyte/erythroid progenitors. (G) A single LT-HSC was sorted into a 96-well plate to perform a colony forming assay. The percentage of colonies was calculated by dividing the original cell number in each colony (n= 3). (H, I) Changes in spleen appearances: long diameter and spleen weight compared with body weight. (J, K) Percentage of LSK cells in Lin⁻ cells (J) and quantification numbers (K) in the spleen (SP). Data are shown as the means ± SEM with at least six samples per group. ^*P < 0.05, ^**P < 0.01 versus respective controls.