Figure 1.

FYY inhibits the proliferation of SMMC-7721 cells. A. FYY inhibited the proliferation of SMMC-7721 cells, but not normal HL-7702 hepatocyte cells, treated for 24, 48, and 72 h in a dose- and time- dependent manner. Cell density and morphology changes are shown (× 100 magnification). B. The colony formation ability was decreased after treatment with FYY (× 100 magnification). C. Representative Western blot and quantitation of PCNA expression showing a dose-dependent decrease with FYY treatment. D. Cell-cycle progression was arrested at the G₂/M phase by FYY. Data are expressed as the means ± SDs from three separate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. PBS.