FYY induces apoptosis of SMMC-7721 cells. Hoechst staining (A) and flow cytometric analysis (B) indicate that FYY induced cellular apoptosis in a dose-dependent manner. (C) Expression levels of apoptosis-related proteins (TRAF2, caspase-8, caspase-3, and PARP) were altered by FYY treatment as determined by Western blotting. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. PBS.