Skip to main content
. 2018 Feb 15;10(2):511–518.

Figure 1.

Figure 1

Enhanced macrophage autophagy is detected in the brains from STZ-treated mice. (A) The beta-cell toxin streptozotocin (STZ) was i.p. injected at a dose of 150 mg/kg body weight to induce high blood glucose in C57BL/6 male at 10 week of age. The control mice (CTL) were injected with saline. Six weeks after STZ injection, the mouse brain was dissociated into single cells to sort F4/80+ macrophages. (B) Representative Western blotting for LC3-II/I, ATG7, p62 and Belcin-1 (C-F) Quantification for LC3-II/I ratio (C), ATG7 (D), p62 (E) and Belcin-1 (F) blotting in macrophages from STZ-treated mice, compared to CTL mice. (G) RT-qPCR for Belcin-1 mRNA in macrophages from STZ-treated mice, compared to CTL mice. *p<0.05. NS: non-significant. N=10.