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. 2018 Feb 15;10(2):511–518.

Figure 2.

Figure 2

Enhanced macrophage autophagy in the brains from STZ-treated mice is likely due to reduced suppression of protein translation of Beclin-1 by miR-384-5p. (A) Bioinformatics show miR-384-5p binding sites on wildtype (wt) 3’-UTR of Beclin-1 mRNA and illustration of mutate (mut) 3’-UTR of Beclin-1 mRNA. (B) RT-qPCR for miR-384-5p levels in brain macrophages. (C) RT-qPCR for miR-384-5p levels in miR-384-5p-modified macrophages. (D, E) Luciferase reporters containing the 3’-UTR of Beclin-1 mRNA (D) or a mutated 3’-UTR of Beclin-1 mRNA in the miR-384-5p binding site (D), as well as miR-384-5p-modifiction plasmids were co-transfected into macrophages to analyze luciferase activity in a dual luciferase assay. *p<0.05. NS: non-significant. N=5.