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. 2018 Feb 15;10(2):592–604.

Figure 4.

Figure 4

CircRNA-000284 directly binds to miR-506 and suppresses miR-506 activity. A: RNA fluorescence in situ hybridization for circRNA-000284. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). B: The putative sequences of miR-506 and circRNA-000284 with two binding sites. C: MiR-506 was upregulated by transfection of miR-506 mimics in cervical cells. D: Luciferase reporter assay was performed to detect the interaction between miR-506 and circRNA-000284. MiR-506 significantly inhibited luciferase activity of wild type reporter for cirRNA-000284, however, miR-506 did not inhibit the luciferase activity of reporter vector containing the mutant binding sites of cirRNA-000284. E: Anti-AGO2 RIP was performed in HeLa cells transfected with miR-506 mimics or NC, followed by RT-qPCR to detect circRNA-000284. F: RT-qPCR experiment suggested that miR-506 was downregulated in cervical cell lines in contrast to normal epithelial cells. G: Cervical cells were transfected by si-circRNAs, then miR-506 expression was detected via RT-qPCR. *P<0.05 vs. NC group; **P<0.01 vs. NC group; ***P<0.001 vs. Input group.