Figure 6.

CircRNA-000284 suppresses cell proliferation and invasion via miR-506/Snail-2 pathway. A: RT-qPCR and Western blot assays suggested that knockdown of circRNA-000284 suppressed Snail-2 expression level at both transcript and protein levels, respectively. B: The sketch of structures of circRNA-000284 is shown. C: RT-qPCR showed that circRNA-000284 expression level was dramatically elevated by the transfection of circRNA-000284 overexpressing vector. D: RIP experiments were performed using the anti-Snail-2 antibody to immunoprecipitate RNA and a primer to detect miR-506, and a significantly decreased enrichment of miR-506 was identified in cells transfected with circRNA-000284 overexpressing vector. E, F: CCK8 assay showed that enhanced expression of circRNA-000284 promoted the cell proliferation rate of both cell lines, however, cotransfection of miR-506 mimics or si-Snail-2 significantly reversed this effect. G: Matrigel invasion assay suggested that the increased cell invasion of cervical cancer cells induced by circRNA-000284 was abrogated by cotransfection with miR-506 mimics or si-Snail-2 vector. *P<0.05; **P<0.01; ***P<0.001 vs. respective control groups.