Table 1.

List of antibodies

Protein Target	Digestion	Primary Antibody	Secondary Antibody
Collagen Type 1	Hyaluronidase (0.5 mg/ml) Acetic Acid (0.5N)	EMD Millipore1 AB755P, 1:200	goat anti-rabbit, BD Biosciences6 550338, 1:200
Collagen Type III	Protease K (0.4 mg/ml) Hyaluronidase (0.5 mg/ml) Acetic Acid (0.5 N)	Sigma-Aldrich2 C7805, 1:500	rat anti-mouse, BD Biosciences 550331, 1:100
Decorin	Chondroitinase-ABC(0.2U/ml) Acetic Acid (0.5 N)	Kerafast3 LF-114, 1:300	goat anti-rabbit, BD Biosciences 550338, 1:200
Fibronectin	Hyaluronidase (0.5 mg/ml)	EMD Millipore AB2040, 1:200	EnVision+HRP anti-rabbit, Agilent Tech.7, undiluted
IL-1β	Pepsin (0.5%)	EMD Millipore AB1832, 1:400	goat anti-rabbit, JacksonImmuno8 111-035-003, 1:100
TNFα	Pepsin (0.5%)	Novus Biologicals4 19532, 1:750	goat anti-rabbit, JacksonImmuno 111-035-003, 1:100
MMP13	Hyaluronidase (0.5 mg/ml)	Santa Cruz Biotech.5, 30073, 1:200	goat anti-rabbit, BD Biosciences 500338, 1:200

IL-1β, interleukin-1-beta; TNFα, tumor necrosis factor alpha; MMP13, matrix metalloproteinase 13

¹Billerica, MA;

²St. Louis, MO;

³Boston, MA;

⁴Littleton, CO;

⁵Dallas, TX;

⁶San Jose, CA;

⁷Santa Clara, CA;

⁸West Grove, PA