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. 2018 Jan 24;19(3):e44871. doi: 10.15252/embr.201744871

Figure EV2. Che‐1 binds c‐Myc promoter and is involved in c‐Myc‐dependent tumorigenesis.

Figure EV2

Related to Fig 4.
  1. Genome Browser Screenshot of Che‐1 promoter, showing E‐box conservation among human and mouse.
  2. qRT–PCR experiment performed in P493‐6 cell line untreated (−tet) or treated with 0.1 μg/ml tetracycline for 72 h (+tet). Values were normalized with actin mRNA levels using the ∆∆C t method. Error bars represent the standard error of three different experiments.
  3. Cross‐linked chromatin derived from P493‐6 cells untreated or treated with 0.1 μg/ml tetracycline for 72 h was immunoprecipitated with c‐Myc antibody or in the absence of antibody and analyzed by qRT–PCR (ChIP–qPCR). Data are expressed as a percentage of input. Error bars represent the standard error of three different experiments.
  4. c‐Myc binds Che‐1 promoter in P493‐6 cell line. Data from Amati's group deposited at GSE51011, UCSC Genome Browser Screenshot showing only portion of MACS2‐generated bigWig.
  5. Che‐1 expression (RPKM) increasing along with tumorigenesis. RNA‐seq processed data from Sabo et al 20 Data are presented as the means and SD from three independent experiments (GSE51011).
Data information: *P ≤ 0.05; **P ≤ 0.01 by Student's t‐test. Source data are available online for this figure.